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Topoisomerase (DNA) II, Binding Protein 1 (TopBP1)

Cat no: T8066-01

Topoisomerase (DNA) II, Binding Protein 1 (TopBP1)

TopBP1 (DNA Topoisomerase II Binding Protein I). a phosphoprotein upregulated during the S phase of the cell cycle, which shares sequence homology with Dpb11 (S.c erevisiae), Rad4 (S. pombe), Mus101 (D. melanogaster) and Cut5 (Xenopus).Studies indicate a role for TopBP1 in DNA replication through interaction with DNA polymerase E, in S phase DNA checkpoint control, and in DNA repair response to ionizing radiation damage. TopBP1 co-localizes with BRCA1 and is an important factor in minimising stalled replication forks and double-strand breaks (DSB), thereby maintaining genomic integrity.\n\nApplications:\nSuitable for use in Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilution: \nImmunoprecipitation: 0.5-2.5ug/mg lysate\nWestern Blot: 1:1000-1:10,000 detects a band of ~200kD in HeLa whole cell lysates.\nOptimal dilution to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8066-01

Size

50ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in Tris-citrate/phosphate and 0.09% sodium azide

P Type

Pab

Purity

Purified by immunoaffinity chromatography

Isotype

IgG

References

1. Yamane, K. et al. (2002) A DNA damage-regulated BRCT-containing protein, TopBP1, is required for cell survival. Mol. Cell Biol. 22: 555-566.

Additional Info

Recognizes human TopBP1 (DNA Topoisomerase II Binding Protein I).

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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