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Topoisomerase (DNA) II, Binding Protein 1 (TopBP1)

Topoisomerase (DNA) II, Binding Protein 1 (TopBP1)

Cat no: T8066-01

Supplier: United States Biological
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TopBP1 (DNA Topoisomerase II Binding Protein I). a phosphoprotein upregulated during the S phase of the cell cycle, which shares sequence homology with Dpb11 (S.c erevisiae), Rad4 (S. pombe), Mus101 (D. melanogaster) and Cut5 (Xenopus).Studies indicate a role for TopBP1 in DNA replication through interaction with DNA polymerase E, in S phase DNA checkpoint control, and in DNA repair response to ionizing radiation damage. TopBP1 co-localizes with BRCA1 and is an important factor in minimising stalled replication forks and double-strand breaks (DSB), thereby maintaining genomic integrity. Applications: Suitable for use in Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Immunoprecipitation: 0.5-2.5ug/mg lysate Western Blot: 1:1000-1:10,000 detects a band of ~200kD in HeLa whole cell lysates. Optimal dilution to be determined by researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: T8066-01
Reactivities: Human
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 50ug
Form: Supplied as a liquid in Tris-citrate/phosphate and 0.09% sodium azide
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography
References: 1. Yamane, K. et al. (2002) A DNA damage-regulated BRCT-containing protein, TopBP1, is required for cell survival. Mol. Cell Biol. 22: 555-566.
Additional info: Recognizes human TopBP1 (DNA Topoisomerase II Binding Protein I).

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