Topoisomerase I, DNA, Recombinant, Human (Scl-70)

DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis. In human tissues the DNA topoisomerase I is initially synthesized as a protein with 100 kD molecular weight. Most of this precursor is then proteolytically processed to a species with 70 kD molecular weight from which the Scl-70 antigen has derived its name. Recombinant Human Scl-70 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 91,576 dalton. Recombinant Scl-70 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8065-02

Size

5ug

Form

Supplied as a liquid in 16mM HEPES, pH 7.5, 400mM sodium chloride, 20% glycerol

Purity

(same/more than)95% (SDS-PAGE)

References

1. A phase I study of the safety and pharmacokinetics of edotecarin (J-107088), a novel topoisomerase I inhibitor, in patients with advanced solid tumors. Cancer Chemother Pharmacol 2006 Jul 4; 2. 4-Nitroquinoline-1-Oxide Induces the Formation of Cellular Topoisomerase I-DNA Cleavage Complexes. Cancer Res 2006 Jul 1;66(13):6540-6545 3. Topoisomerase I, II{alpha} and II{beta} mRNA expression in peripheral blood mononuclear cells of patients with solid tumor: preliminary results. Ann Oncol 2006 May;17 Suppl 5:v25-v28 4. Cellular stress triggers the human topoisomerase I damage response independently of DNA damage in a p53 controlled manner. Oncogene 2006 Jun 26; 5. DNA topoisomerase I from parasitic protozoa: A potential target for chemotherapy. Biochim Biophys Acta 2006 Mar-Apr;1759(3-4):117-31 6. Evaluation of indenoisoquinoline topoisomerase I inhibitors using a hollow fiber assay. Bioorg Med Chem Lett 2006 May 31;