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Topoisomerase II (DNA) alpha (DNA Topoisomerase 2 alpha, DNA Topoisomerase II alpha Isozyme, TOP2, TOP2A, TP2A)

Topoisomerase II (DNA) alpha (DNA Topoisomerase 2 alpha, DNA Topoisomerase II alpha Isozyme, TOP2, TOP2A, TP2A)

Cat no: T8065-12F

Supplier: United States Biological
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DNA topoisomerase 2 catalyzes the transient breaking and rejoining of double stranded DNA during events such as DNA synthesis and transcription. Two forms of this enzyme exist, alpha and beta. DNA topoisomerase 2 alpha (TOP2A) is localized to chromosome 17 and the beta gene is localized to chromosome 3. At least 4 isoforms of TOP2A are produced by alternate splicing. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: T8065-12F
Reactivities: Human, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul of sterile dH2O.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: DNA Topoisomerase 2-alpha, EC=5.99.1.3, Topoisomerase (DNA) II alpha 170kDa
Additional info: Recognizes human DNA topoisomerase 2-alpha at 174kD. Species Crossreactivity: rat.

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