Topoisomerase II (DNA) alpha

Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.\n\nApplications:\nSuitable for use in ELISA and Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilution:\nImmunohistochemistry (Formalin/paraffin):1:20 for 20 min at RT. Antigen retrieval of boiling in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at RT for 20 min is required. Optimal dilutions to be determined by researcher. \n\nPositive Control: Tonsil\n\nCellular Localization: Nuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8065-14

Size

500ul

Applications

ELISA, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.09% sodium azide before the addition of glycerol to 40%.

P Type

Mab

Purity

Culture supernatant.

Isotype

IgG2a,k

References

1. Kreipe H, et al. (1993) Am J Pathol, 142:3-9.\n2. Rudolph P, et al. (1995) Am J Pathol, 147:1615.\n3. Kreipe H, et al. (1993) Am J Pathol, 142:651-657.\n4. Claviez A, et al. (1994) Ann Hematol, 68:61-66.\n5. Woessner r D, et al. (1991) Cell Growth Differ, 2:209-214.\n6. Boege F, et al. (1995) Am J Pathol, 146:1302-1308.

Additional Info

Recognizes Topoisomerase II alpha. Species Crossreactivity: Human.