Toso, CT (Regulator of Fas-induced Apoptosis, Fas Apoptotic Inhibitory Molecule 3, FAIM3)

Toso, also known as Fas Apoptotic Inhibitory Molecule 3 (FAIM3). Toso is expressed by lymphoid and hematopoietic cells and is up-regulated by T-cell activation. Toso expressing cells have increased resistance to Fas-, FADD-, and TNFalpha-induced apoptosis. Toso is membrane bound, and is thought to directly bind to FADD, disrupting DISC formation and thus inhibiting caspase-8 activation. Toso is a potential proto-oncogene, and could be a therapeutic target for use in the treatment of autoimmune diseases. Despite its predicted molecular weight, Toso often migrates at 60kD in SDS PAGE.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml detects a band of ~43kD in human lung tissue lysate.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T8067-50C

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hitoshi, Y. et al. (1998) Toso, a cell surface, specific regulator of Fas-induced apoptosis in T cells. Immunity. 8: 461-471. 2. Song, Y. and Jacob, C.O. (2005) The mouse cell surface protein TOSO regulates Fas/Fas ligand-induced apoptosis through its binding to Fas-associated death domain. J. Biol. Chem. 280: 9618-9626.

Additional Info

Recognizes an epitope within the C-terminal region of human Toso. Species sequence homology: mouse, rat.