Toxoplasma gondii (Major Surface Antigen p30)

Toxoplasma gondii is a protozoan parasite that can infect healthy individuals and also cause congenital defects in the fetus and severe infection in immunocom- promised hosts. This antibody will help detect most strains of Toxoplasma gondii as it recognizes the highly conserved and prominent P30 surface antigen.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry: 1:10-1:20 for 1 hour at RT. Requires antigen retrieval.\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls: \nToxoplasma gondii-infected cerebellum. Cytoplasm of infected cells.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8076-35B

Size

1ml

Applications

IHC

Hosts

Mouse

Form

Supplied as a lyophilized powder from PBS, 1% BSA, 15mM sodium azide. Reconstitute with 1ml sterile ddH2O.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Decoster A, Gontier P, Dehecq E, et al.. Detection of anti-Toxoplasma immunoglobulin A antibodies by Platelia-Toxo IgA directed against P30 and by IMx Toxo IgA for diagnosis of acquired and congenital toxoplasmosis. Journal of Clinical Microbiology. 33(8):2206-2208 (1995). 2.Bonhomme A, Thirion C, Boulanger F, et al.. Toxoplasma gondii-structure variations of the antigen P30. Parasitology. 108:281-287 (1994). 3.Godard I, Estaquier J, Zenner L, et al.. Antigenicity and immunogenicity of P30-derived peptides in experimental models of toxoplasmosis. Molecular Immunology. 31(17):1353-1363 (1994). 4.Kim K, B ?low R, Kampmeier J et al.. Conformationally appropriate expression of the Toxoplasma antigen SAG1 (p30) in CHO cells. Infection and Immunity. American Society for Microbiology. 62(1):203-209 (1994). 5. Mineo J R, McLeod R, Mack D et al.. Antibodies to Toxoplasma gondii major surface protein (SAG-1, P30) inhibit infection of host cells and are pro- duced in murine intestine after peroral infection. The Journal of Immunology. 150(9):3951-3964 (1993).

Additional Info

Recognizes Toxoplasma gondii P30 Antigen.