Toxoplasma gondii, MIC 3, aa234-306, Recombinant

Toxoplasma is a crescent shaped sporozoan that lives as intracellular parasite in various tissues of vertebrates and completes its life cycle in a single host. It is transmitted via raw/undercooked meat, contaminated soil, or by direct contact. Infection due to Toxoplasma gondii usually occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy following organ transplant. Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.\n\nThe protein contains the MIC 3 immunodominant regions, amino acids: (234-306).\n\nSpecificity:\nImmunoreactive with sera of Toxoplasma gondii-infected individuals.\n\nApplications:\nAntigen in ELISA and Western Blot, excellent antigen for detection of Toxoplasma gondii- with minimal specificity problems.\n\nRecommended Dilutions: Optimal dilutions to be determined by the reasearcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8076-32A

Size

100ug

Form

Supplied as a liquid in 50mM Tris-HCl, 1.5M urea, 50% glycerol.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. The MIC3 gene of Toxoplasma gondii is a novel potent vaccine candidate against toxoplasmosis. Ismael AB, Sekkai D, Bout D, Infect Immun 2003 Nov;71(11):6222-8 2. The Toxoplasma gondii protein MIC3 requires pro-peptide cleavage and dimerization to function as adhesin. Cerede O, Dubremetz JF, Lebrun M, EMBO J 2002 Jun 3;21(11):2526-36 3. The microneme protein MIC3 of Toxoplasma gondii is a secretory adhesin that binds to both the surface of the host cells and the surface of the parasite. Garcia-Reguet N, Lebrun M, Mercereau-Puijalon O, Van Beeumen J, Cell Microbiol 2000 Aug;2(4):353-64.