Toxoplasma gondii, P24 (GRA1), aa57-149, Recombinant

Toxoplasma is a crescent shaped sporozoan that lives as intracellular parasite in various tissues of vertebrates and completes its life cycle in a single host. It is transmitted via raw/undercooked meat, contaminated soil, or by direct contact. Infection due to Toxoplasma gondii usually occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy following organ transplant. Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.\n\nThe protein contains the P24 (GRA1) immunodominant regions, amino acids: (57-149).\n\nSpecificity:\nImmunoreactive with sera of Toxoplasma gondii-infected individuals.\n\nApplications:\nAntigen in ELISA and Western Blot, excellent antigen for detection of Toxoplasma gondii- with minimal specificity problems.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T8076-33A

Size

100ug

Form

Supplied as a liquid in 50mM Tris-HCl, 1.5M urea, 50% glycerol.

Purity

(same/more than) 95% by 10% PAGE (coomassie staining)

References

1. [Construction and sequencing of recombinant plasmid pcDNA3/GRA1 from Toxoplasma gondii] Cai LT, Shu HP, Hunan Yi Ke Da Xue Xue Bao 2003 Jun;28(3):221-3 2. Generation of Toxoplasma gondii GRA1 protein and DNA vaccine loaded chitosan particles: preparation, characterization, and preliminary in vivo studies. Bivas-Benita M, Laloup M, Dewit J, Int J Pharm 2003 Nov 6;266(1-2):17-27 3. [A comparative study of GRA1 gene between the two strains of different virulence of Toxoplasma gondii] Liu CM, Tan DM, Hunan Yi Ke Da Xue Xue Bao 2000 Dec 28;25(6):587-8 4. High yield expression and single-step purification of Toxoplasma gondii SAG1, GRA1, and GRA7 antigens in Escherichia coli. Hiszczynska-Sawicka E, Brillowska-Dabrowska A, Pietkiewicz H, Protein Expr Purif 2003 Jan;27(1):150-7 5. A GRA1 DNA vaccine primes cytolytic CD8(+) T cells to control acute Toxoplasma gondii infection. Scorza T, D'Souza S, Dewit J, Huygen K, Infect Immun 2003 Jan;71(1):309-16 6. Identification of a human immunodominant B-cell epitope within the GRA1 antigen of Toxoplasma gondii by phage display of cDNA libraries. Beghetto E, Pucci A, Spadoni A, Felici F, Int J Parasitol 2001 Dec;31(14):1659-68.