Toxoplasma gondii, P30 (SAG1), aa45-198, Recombinant

The life cycle of Toxoplasma gondii has two phases. The coccidia like takes place only in members of the Felidae family which makes these animals the parasite's primary host. The asexual part of the life cycle can take place in any warm-blooded animal, like other mammals (including felines) and birds. T. gondii constructing daughter scaffolds within the mother cell. In the intermediate hosts (including felines), the parasite invades cells, forming intracellular so-called parasitophorous vacuoles containing bradyzoites, the slowly replicating form of the parasite. Vacuoles form tissue cysts mainly within the muscles and brain. Since they are within cells, the host's immune system does not detect these cysts. Resistance to antibiotics varies, but the cysts are very difficult to eradicate entirely. Within these vacuoles T. gondii propagates by a series of binary fissions until the infected cell eventually bursts and tachyzoites are released. Tachyzoites are the motile, asexually reproducing form of the parasite. Unlike the bradyzoites, the free tachyzoites are usually efficiently cleared by the host's immune response, although some manage to infect cells and form bradyzoites, thus maintaining the infection.\n\nSpecificity:\nImmunoreactive with sera of Toxoplasma gondii-infected individuals.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8076-38A

Size

100ug

Form

Supplied as a liquid in 50mM Tris-HCl, 1.5M urea, 50% glycerol.

Purity

(same/more than) 95% by 10% PAGE (coomassie staining)

References

1. Robinson, S.A. & Smith, J.E., Parasitology 128(4): 391-396 (2004). 2. Seng, S., et al., Pathobiology 71(3):144-151 (2004). 3. Zheng, D.L., et al., Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 21(4): 230-233 (2003). 4. Chen, H., et al., Chin. Med. J. (Engl.) 116(10): 1561-1566 (2003). 5. Sager, H., et al., Parasitol. Res. 91(2): 171-174 (2003). 6. Mohamed, R.M., et al., Vaccine 21(21-22): 2852-2861 (2003).