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TP53INP2, CT (Tumor Protein p53-inducible Nuclear Protein 2, p53-inducible Protein U, PIG-U, PINH, C20orf110, DOR) (FITC)

Cat no: T5501-20-FITC

TP53INP2, CT (Tumor Protein p53-inducible Nuclear Protein 2, p53-inducible Protein U, PIG-U, PINH, C20orf110, DOR) (FITC)

TP53INP2/DOR is a scaffold protein that recruits LC3 and/or LC3-related proteins to the autophagosome membrane by interacting with the transmembrane protein VMP1. TP53INP2 translocates from the nucleus to the autophagosome structures after activation of autophagy by rapamycin or starvation. TP53INP2 is necessary for autophagosome development because its small interfering RNA-mediated knockdown strongly decreases sensitivity of mammalian cells to autophagy.\n\nApplications: \nSuitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nFlow Cytometry: 1:10-1:50\nImmunohistochemistry: 1:50-1:100\nWestern Blot: 1:100-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze FITC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T5501-20-FITC

Size

200ul

Applications

ELISA, FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to FITC.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Bennetts,J.S., et.al., Dev. Dyn. 236 (3), 843-852 (2007). 2.Jonathan Nowak, Cendrine Archange,et.al., Molecular Biology of the Cell Vol. 20, 870-881, February 1, 2009. 3.Bernhard G. Baumgartner, et.al., Plos one: November 2007 Issue 11 e1183.

Additional Info

Recognizes human TP53INP2. Species Crossreactivity: mouse.

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