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TPSAB1, Recombinant, Human, aa31-275, His-Tag (Tryptase Alpha/beta-1, TPS1, TPS2, TPSB1)

Cat no: 138764

TPSAB1, Recombinant, Human, aa31-275, His-Tag (Tryptase Alpha/beta-1, TPS1, TPS2, TPSB1)

TPSAB1, also known as tryptase alpha/beta-1, is a tryptase that is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. It is enzymatically active only as a heparin-stabilized tetramer, and it is resistant to all known an endogenous proteinase inhibitor. This protein has been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. \n\nSource:\nRecombinant protein corresponding to aa31-275 from human TPSAB1 at N-terminal, fused to His-tag expressed in E.coli. \n\nMolecular Weight: \n~30.1kD (270aa)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSHM YYQDQLLPVS RIIVHPQFYT AQIGADIALL ELEEPVNVSS HVHTVTLPPA SETFPPGMPC WVTGWGDVDN DERLPPPFPL KQVKVPIMEN IVGGQ EAPRSKWPWQ VSLRVHGPYW MHFCGGSLIH PQWVLTAAHC VGPDVKDLAA LRVQLREQHL HICDAKYHLG AYTGDDVRIV RDDMLCAGNT RRDSCQGDSG GPLVCKVNGT WLQAGVVSWG EGCAQPNRPG IYTRVTYYLD WIHHYVPKKP\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

138764

Size

50ug

Form

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.4M urea,10% glycerol.

Purity

~85% (SDS-PAGE)

References

1. Marquardt U. et al. (2002) J Mol Biol. 321(3):491-502. 2. Tanaka T. et al. (1983) J Biol Chem. 258(22):13552-7.

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