TPX2 (P100, C20orf1, C20orf2, DIL2, HCA519, p100, Protein FLS353, Hepatocellular carcinoma-associated antigen 519, Targeting protein for Xklp2, Restricted expression proliferation associated protein 100, Differentially expressed in cancerous and noncancer

The nuclear protein p100 is a proliferation-associated protein whose expression is restricted to cell cycle phases S, G2, and M. Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. The full-length cDNA encodes a 747aa protein with a putative ATP/GTP-binding site motif. RT-PCR analysis demonstrated strong expression of in lung carcinoma cell lines but not in normal lung tissues. Expression was also found in adult placenta, skeletal muscle, thymus, testis, and small intestine and in fetal brain, liver, and kidney. P100 is also correlated to cancer prognosis.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000 \nWestern Blot: 1:100-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze horseradish peroxidase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8100-05-HRP

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1.Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). 2.Heidebrecht, H.J., et al., Mol. Cancer Res. 1(4):271-279 (2003). 3.Garrett, S., et al., Curr. Biol. 12(23):2055-2059 (2002). 4.Gruss, O.J., et al., Nat. Cell Biol. 4(11):871-879 (2002). 5.Kufer, T.A., et al., J. Cell Biol. 158(4):617-623 (2002).

Additional Info

Recognizes human P100. Species Crossreactivity: mouse.