TRA-1-60 (TRA-1, Tumor Rejection Antigen 1) (PE)

TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells. The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors. Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan. Subsequent \nevidence implicated podocalyxin as a carrier for the TRA-1-60 epitope. The original TRA-1-60 antibody is now known to include two distinct specificities that recognize a neuraminidase resistant epitope [TRA-1-60(R)] or a neuraminidase-sensitive epitope [TRA-1-60(S)]. \n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: Designed to quantitatively determine the percentage of cells bearing TRA-1-60(R) within a population and qualitatively determine the density of TRA-1-60(R) on cell surfaces by flow cytometry. Cell surface expression of TRA-1-60(R) is determined by flow cytometry using 488 nm wavelength excitation and monitoring emitted fluorescence with a detector BG01V cells were stained with PE-conjugated anti-human TRA-1-6 (filled histogram) or isotype control (open histogram). \nOptimal dilutions to be determined by the researcher.\n \nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8115A

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in 25ug antibody, 1ml saline, 0.5% BSA, 0.1% sodium azide.

P Type

Mab

Purity

Human embryonal carcinoma cell line, 2102E9

Isotype

IgM

References

1. Andrews, P. et al. (1984) Hybridoma 3:347. 2. Thomson, J. et al. (1998) Science 282:1145. 3. Giwercman, A. et al. (1993) Cancer 72:1308. 4. Marrink, J. et al. (1991) Int. J. Cancer 49:368. 5. Badcock, G. et al. (1999) Cancer Res. 59:4715. 6. Schopperle, W. & W. DeWolf (2007) Stem Cells 25:723.