TRA-1-60 specific antibody reacts with podocalyxin, a sialomucin which is a member of the CD34 family. It is expressed on human teratocarcinoma cells (EC), embryonic stem (ES) and germ cells. It is not expressed on differentiated cells. TRA-1-60R is a neauraminidase-resistant epitope variant of TRA-1-60. TRA-1-60 expression is proportional to tumor growth.
Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 0.5-1ug labels 10e6 cells
Immunohistochemistry: Formalin fixed paraffin embedded
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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