TRAF Interacting Protein TANK (CT) (TRAF Family Member-associated NFkB Activator)

TANK was initially identified as a novel TRAF-interacting protein that regulated TRAF-mediated signal transduction. Specifically, ligand binding by surface receptors in the tumor necrosis factor (TNF) receptor and Toll/interleukin-1 (IL-1) receptor families lead to the formation of a TRAF/TANK complex that mediates the activation of the transcription factor NF-kappaB. At least 2 isoforms of TANK are known to exist.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8171-79C

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Cheng, G., et al., (1996), \"TANK, a co-inducer with TRAF2 of TNF- and CD40L-mediated NF-kappaB activation\", Genes Dev., 10: 963-973. 2. Rothe, M., et al., (1996), \"I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction\", Proc. Natl. Acad. Sci. USA, 93: 8241-8246. 3. Pomerantz, J.L., et al., (1999), \"NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase\", EMBO J., 18: 6694-6704. 4. Chariot, A., et al., (2002), \"Association of the adaptor TANK with the I kappaB kinase (IKK) regulator NEMO connects IKK complexes with the IKK epsilon and TBK1 kinases\", J. Biol. Chem., 277: 37029-37036.

Additional Info

Recognizes human TANK, C-terminal. Species Crossreactivity: mouse, rat.