TRAF Interacting Protein TANK (TANK, I TRAF, ITRAF, I-TRAF, TRAF Family Member-associated NF kappa B Activator, TRAF Family Member-associated NFkB Activator, TRAF-interacting Protein, TRAF-interacting Protein TANK Isoform a, TRAF-interacting Protein TANK

The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor (TNFR) superfamily. TANK:I-TRAF (TRAF family member associated NF-kB activator:TRAF-interacting protein) that was first defined as a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction (reviewed in Bonif, 2006). TANK:I-TRAF is thought to inhibit TRAF function by sequestering the TRAFs in the cytoplasm, thereby blocking their recruitment to the TNFR. TANK:I-TRAF has also been shown to bind to other proteins in the NF-kB signaling pathway, including NEMO. However, the role and different functions of TANK:I-TRAF in NF-kB signaling pathways remains to be fully elucidated. Recognizes TANK:I-TRAF, human TANK:I-TRAF is a 425aa protein.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:2000\nImmunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000\nImmunoprecipitation: 1:50-1:200\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nLymphatic tissues, many cancer cell lines\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8171-75C

Size

50ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Bonif M, M-A Meuwis, P Close, V Benoit, K Heyninck, J-P Chapelle, V Bours, M-P Mervile, J Piette, R Beyaert, and A Chariot. 2006. TNFa- and IKKb-mediated TANK/I-TRAF phosphorylation: implications for interaction with NEMO/IKKg and NF-kB activation. Biochem J 394:593-603.

Additional Info

Recognizes human TANK/I-TRAF. Species sequence homology: mouse, rat, bovine.