e range of cell surface receptors, participating in the regulation of cell survival, proliferation, differentiation and stress responses. TRAF-associated NF- B activator (TANK), also known as TRAF-interacting protein (I-TRAF), is a TRAF binding protein that demonstrates both stimulatory and inhibitory properties (5,6). TANK binds to the carboxy domain of the TRAF1, -2 and -3. Overexpression of TANK prevents the association of TRAF2 with TNFR2, inhibiting TNFR2 and CD40 induced NF- B activation (6). TANK is also reported to synergize with low levels of TRAF2 to activate NF- B (5). TANK assists in the activation of NF- B via association and activation of TANK-binding kinase 1 (TBK1) or IKK which promotes activation of the IKK complex (7,8). It has also been shown that TANK may synergize with TRAF2, TRAF5, and TRAF6 but not TRAF3 in SAPK activation (9). TNF stimulation results in IKK -dependent phosphorylation of TANK which may provide negative feedback regulation of TANK mediated NF- B activation (10).
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions:
Immunohistochemistry: 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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