TRAF Interacting Protein TANK (TRAF Family Member-associated NFkB Activator)

TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins, forming multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy terminus "TRAF domain" which mediates interactions with associated proteins and many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). At least six TRAFs have been identified (TRAF1-6) and they have been found to act as adaptor proteins for a wide range of cell surface receptors, participating in the regulation of cell survival, proliferation, differentiation and stress responses. TRAF-associated NF- B activator (TANK), also known as TRAF-interacting protein (I-TRAF), is a TRAF binding protein that demonstrates both stimulatory and inhibitory properties (5,6). TANK binds to the carboxy domain of the TRAF1, -2 and -3. Overexpression of TANK prevents the association of TRAF2 with TNFR2, inhibiting TNFR2 and CD40 induced NF- B activation (6). TANK is also reported to synergize with low levels of TRAF2 to activate NF- B (5). TANK assists in the activation of NF- B via association and activation of TANK-binding kinase 1 (TBK1) or IKK which promotes activation of the IKK complex (7,8). It has also been shown that TANK may synergize with TRAF2, TRAF5, and TRAF6 but not TRAF3 in SAPK activation (9). TNF stimulation results in IKK -dependent phosphorylation of TANK which may provide negative feedback regulation of TANK mediated NF- B activation (10).\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern blotting 1:1000\nImmunoprecipitation 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8171-79B

Size

100ul

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

(1) Arch, R.H. et al. (1998) Genes Dev. 12, 2821

Additional Info

Detects endogenous levels of total TANK protein. It does not crossreact with other TRAF family members. Species Crossreactivity: Bovine, human, monkey, mouse, and rat.