TRAF5 (TNF Receptor Associated Factor 5, TNF Receptor-associated Factor 5, TRAF 5, TRAF-5, MGC-39780, RING Finger Protein 84, RNF84)

Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6) (1-4). TRAF5 is a 558-amino acid protein. TRAF5 is implicated in NF-kB and c-Jun NH(2)-terminal kinase/stress-activated protein kinase activation by members of the TNF receptor superfamily, including CD27, CD30, CD40, and lymphotoxin-beta receptor . Targeted disruption of TRAF5 gene causes defects in CD40-CD27 mediated lymhocyte activation (5).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Cytoplasmic (probable)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8172-50A

Size

50ug

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Nakano H, Sakon S, Koseki H, Takemori T, Tada K, Matsumoto M, Munechika E, Sakai T, Akiba H, Kobata T, Santee SM, Ware CF, Rennert PD, Taniguchi M, Yagita H., and Okumura K. Proc. Natl. Acad. Sci. USA 96 (17): 9803-9808 (1999). \n2. Cao Z, Xiong J, Takeuchi M, and Kurama, T. Nature 383: 443-446 (1996). \n3. Rothe M, Wong SC, Henzel WJ, and Goeddel DV. Cell 78: 681-692 (1994). \n4. Cheng G, Cleary AM, Ye ZS, Hong DI, Lederman S, and Baltimore D. Science 267: 1494-1498 (1995).\n5. Nakane H, Oshima H, Chung W, Williams-Abbit L, Ware CF, Yagita H, and Okumura K. J. Biol. Chem. 271: 14661-14664 (1996).

Additional Info

In HeLa, a ~64kD band is observed. Species Crossreactivity: Crossreacts with Human.