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TRAF6 (TNF Receptor-associated Factor 6, E3 Ubiquitin-protein Ligase TRAF6, Interleukin-1 Signal Transducer, MGC:3310, RING Finger Protein 85, RNF85)

Cat no: T8172-60B

TRAF6 (TNF Receptor-associated Factor 6, E3 Ubiquitin-protein Ligase TRAF6, Interleukin-1 Signal Transducer, MGC:3310, RING Finger Protein 85, RNF85)

Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-kB. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK.\n\nApplications:\nSuitable for use in Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:3000\nImmunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml\nImmunofluorescence: 1:100-1:200\nImmunocytochemistry: 1:100-1:200\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8172-60B

Size

100ul

Applications

ICC, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7, 0.01% thimerosal, 10% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Recognizes human TRAF6. Species sequence homology: bovine (82%), porcine (83%) and monkey (97%).

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