TRAIL, aa261-277 (TNF-Related Apoptosis Inducing Ligand, Apo2L, TL2, TNFSF10)

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel member in the TNF family was recently identified and designated TRAIL (for TNF-related apoptosis-inducing ligand) and Apo-2L (for Apo-2 ligand)1,2. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Two novel death domain containing receptors DR4 and DR5 have been identified as the receptor for TRAIL3-6. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.\n\nThis polyclonal antibody can be used for detection of TRAIL expression by Western blot at 1:500 dilution. Whole cell lysate from HeLa cells can be used as positive control and a 32kD band, which represents a membrane bound form of TRAIL, should be detected.\n\nWe have only tested TRAIL in human with Western blot. The 17 residues of\nTRAIL immunogenic peptide has 83% identity and 93% homology to mouse\nhomologue. It has good chance to be mouse reactive.\n\n

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SPECIFICATIONS

Catalog Number

T8175

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified IgG

Isotype

IgG

References

1. Wiley SR, et al . Immunity 1995;3:673-682 2. Pitti RM; et al . J. Biol. Chem. 1996; 271:12687-903. Pan G;et al. Science; 1997;276:111-1134. Schneider P, et al. Immunity 1997;7:831-836 5. Pan G, et al.,. Science 1997;277:815-818 6. Sheridan JP, et al. Science 1997;277:818-821

Additional Info

A 32kD band, which represents a membrane bound form of TRAIL, should be detected.