TRAIL, Recombinant, Human (TNF-Related Apoptosis Inducing Ligand, Apo2L, TL2, TNFSF10)

TRAIL, 114-281 aa, Human, Recombinant, E. coli\n\nRecombinant human TRAIL was expressed in E.coli and purified by using conventional chromatography techniques.\n\nTNF-related apoptosis-inducing ligand (TRAIL) belongs to the tumor necrosis factor (TNF) cytokine family and induces rapid apoptosis in a wide variety of tumor cell lines upon binding to the death-signalling receptors on the cell membrane. TRAIL binds to one of four receptors that have been identified in humans, including TRAIL-R1/DR4, TRAIL-R2/KILLER/DR5, TRAIL-R3/DcR1/TRID, and TRAIL-R4/DcR2/TRUNDD. Both DR4 and DR5 are pro-apoptotic receptors, which contain a cytoplasmic death domain and mediate apoptosis on binding to TRAIL. By contrast, TRID and TRUNDD do not contain a cytoplasmic death domain and block the function of TRAIL by competing with DR4 and DR5 for binding of TRAIL.\n\nMolecular Weight: 23kD (211 aa)\n\nAmino Acid Sequence: \nMVRERGPQRV AAHITGTRGR SNTLSSPNSK NEKALGRKIN SWESSRSGHS FLSNLHLRNG\nELVIHEKGFY YIYSQTYFRF QEEIKENTKN DKQMVQYIYK YTSYPDPILL MKSARNSCWS\nKDAEYGLYSI YQGGIFELKE NDRIFVSVTN EHLIDMDHEA SFFGAFLVG\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

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SPECIFICATIONS

Catalog Number

T8180-15B

Size

50ug

Form

Supplied as a liquid in 20mM Tris pH 7.5, 300mM NaCl, 0.1mM DTT, 10% glycerol.

Purity

(same/more than) 95% by SDS-PAGE

References

1. Human Brain Astrocytes Mediate TRAIL-mediated Apoptosis after Treatment with IFN-gamma. Yonsei Med J 2006 Jun 30;47(3):354-8\n2. [Influence of Chemotherapy on Expression of TRAIL in Primary Acute Leukemic Cells.] Zhongguo Shi Yan Xue Ye Xue Za Zhi 2006 Jun;14(3):481-4\n3. Improvement of Expression Level and Bioactivity of Soluble Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (Apo2L/TRAIL) by a Novel Zinc ion Feeding Strategy Lett 2006 Jun 24;\n4. The antitumor activity of TRAIL and IL-24 with replicating oncolytic adenovirus in colorectal cancer. Cancer Gene Ther 2006 Jun 23;\n5. TRAIL-induced apoptosis proceeding from caspase-3-dependent and -independent pathways in distinct HeLa cells. Biochem Biophys Res Commun 2006 Jun 12;\n6. [Effects of hTERT RNAi on apoptosis of hepatocellular carcinoma cells induced by TRAIL.] Zhonghua Gan Zang Bing Za Zhi 2006 Jun;14(6):435-40\n7. Janssen EM, et al , (2005). Nature. Mar 3;434(7029):88-93\n8. Nagane M , et al , (2001). Apoptosis. Jun;6(3):191-7.\n