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TRAIL, Recombinant, Mouse (TNF-related apoptosis-inducing Ligand, TNFSF10, TL2)

Cat no: 143529

TRAIL, Recombinant, Mouse (TNF-related apoptosis-inducing Ligand, TNFSF10, TL2)

TRAIL is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells.TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5.These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic death domainrdquo, which activates the cell's apoptotic machinery.Recombinant murine TRAIL is a 174 amino acid polypeptide (20.0kD), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL protein.\n\nBiological Activity:\nAssay#1: Determined by the dose-dependent stimulation of MIP-2 production by mouse spleen cells using a concentration range of 10-100ng/ml.\nAssay#2:Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 40.0-60.0ng/ml.\n\nAA Sequence:\nMRGGRPQKVAAHITGITRRSNSALIPISKDGKTLGQKIESWESSRKGHSFLNHVLFRNGELVIEQEGLYYIYSQTYFRFQEAEDASKMVSKDKVRTKQLVQYIYKYTSYPDPIVLMKSARNSCWSRDAEYGLYSIYQGGLFELKKNDRIFVSVTNEHLMDLDQEASFFGAFLIN\n\nCrossreactivity:\nHuman\n\nQuality Control:\nVerified by N-terminal and Mass Spectrometry analyses (when applicable).\n\nEndotoxin:\n<0.1ng/ug of protein (<1EU/ug).\n\nProtein Content:\nVerified by UV Spectroscopy and/or SDS-PAGE gel.\n\nStorage and Stability:\nStore lyophilized products at -20 degrees C. For reconstituted solutions of most products, we recommend short-term storage at 4 degrees C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20 degrees C. Additional freeze/thaw cycles may cause some denaturation of the protein.

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SPECIFICATIONS

Catalog Number

143529

Size

10ug

Form

Supplied as a lyophilized powder.

Purity

(same/more than)95% by SDS-PAGE gel and HPLC analyses.

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