TRAIL (TNF Related Apoptosis Inducing Ligand, TNF-related Apoptosis-inducing Ligand TRAIL, Apo 2 Ligand, Apo2 Ligand, Apo-2 Ligand, Apo 2L, Apo2L, Apo-2L, CD253, CD253 Antigen, Protein TRAIL, TL2, Tumor Necrosis Factor (Ligand) Superfamily Member 10, TNFS

Specific for human CD253, otherwise known as TRAIL or Apo2L, a 281aa type II transmembrane glycoprotein and member of the tumor necrosis factor superfamily, which induces apoptosis in tumor and transformed cells, but not normal cells. \n\nActivation of apoptosis by CD253 occurs through binding to the death-signaling receptors DR4 (TRAILR1) and DR5 (TRAILR2), leading to formation of the death-inducing signaling complex (DISC) and the triggering of a proteolytic caspase cascade. There are however decoy receptors known as DcR1 (TRAILR3) and DcR2 (TRAILR4) which competitively bind to CD253 and are unable to initiate apoptosis. \n\nCD253 also plays a role in immune surveillance, acting as a mediator of monocyte, NK cell, dendritic cell and T cell anti-tumor and anti-viral cytotoxicity.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nELISA: 0.15-0.3ug/ml \nWestern Blot: 0.1-0.2ug/ml\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C for short-term only.Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T8180-01M

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a lyophilized powder from PBS. No preservative added. Labeled with Biotin. Reconstitute with 500ul sterile 40-50% glycerol, 0.1% BSA, PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Almasan, A. and Ashkenazi, A. (2003) Apo2L/TRAIL: apoptosis signaling, biology and potential for cancer therapy. Cytokine Growth Factor Rev. 14: 337-348.

Additional Info

Recognizes human CD253.