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Transcobalamin II (TCN2) ELISA Kit

Cat no: ABIN422422


Supplier: Antibodies-online
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Transcobalamin II (TCN2) ELISA Kit: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcobalamin II, Macrocytic Anemia (TCN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcobalamin II, Macrocytic Anemia (TCN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcobalamin II, Macrocytic Anemia (TCN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm
Catalogue number: ABIN422422
Size: 96 tests
Accession: P20062
Gene: 6948
Antigen: TCN2
Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range: 0.156-10 ng/mL
Sample type: Serum,Plasma,Biological Fluids
Entrez gene: 6948

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