Transducin-Like Enhancer of Split 1 (TLE1, E(Sp1) Homolog, Enhancer of Split Groucho-like Protein 1, ESG, ESG1, GRG1, Transducin-like Enhancer Protein 1)

TLE1 (Transducin-Like Enhancer of Split 1; also ESG1 and GRG1) is a member of the WD repeat Groucho/TLE family of transcriptional repressors. It is widely expressed, and is known to antagonize NFkB and TCF (Wnt)-mediated signaling. TLE1 binds to other family members as a heterooligomer, or to itself as a homooligomer. While it possesses no intrinsic DNA-binding activity, it does modulate the activity of bound transcription factors. Human TLE1 is 770 amino acids (aa) in length. It contains a Gln-rich region that mediates oligomerization (aa1-131), a CCN domain that contains an NLS and CDC2 kinase site (aa200-268), and six WD repeats that mediate protein-protein interaction (aa470-767). Phosphorylation on Ser will generate 90-93kD and 116kD forms in SDS-Page. Potential splice variants exist. There are alternative start sites at Met57 and Met326, a 10aa insertion after Gly124, and three distinct eight aa substitutions for aa 193-770, 193-199 and 445-770. Over aa11-200, human TLE1 shares 98% aa identity with mouse TLE1. \n \nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml. The detection limit for rhTLE1 is approximately 2ng/well. \nWestern Blot: 2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8197-93

Size

100ug

Applications

ELISA, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophillized power in PBS, 5% Trehalose. Reconstitute with sterile 40-50% glycerol, PBS.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human TLE1. In direct ELISAs, this antibody shows ~10% cross-reactivity with rhTLE3 and rhTLE4 and less than 5% cross-reactivity with rhTLE2.