Transferrin, Human, HOLO, 95+% (Iron-saturated Siderophilin, TRF)

Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of ~79.5kD and a serum concentration range of 1800-2700mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.\n\nHuman Transferrin is a glycoprotein with homologous N-terminal and C-terminal iron binding domains. Transferrin(TF) is related to other iron binding proteins including lactoferrin. When human transferrin loaded with iron encounters a transferrin receptor on the surface of a cell, it binds to it and is consequently transported into the cell in a vesicle. The cell will acidify the vesicle, causing human transferrin(TF) to release its iron ions. Each human transferrin molecule has the ability to carry two iron ions in the ferric form (Fe3+).\n\nHuman Transferrin levels may be checked in iron deficiency, hemochromatosis and other iron overload disorders. Transferrin is also associated with the innate immune system. Human Transferrin is found in the mucosa and binds iron, thus creating an environment low in free iron, where few bacteria are able to survive.\n\nSource:\nTransferrin, from human plasma.\n\nIron content: (same/more than)1ug/mg protein \n \nTotal Iron Binding Capacity: 1.2-1.6ug/mg\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T8199-12C

Size

100mg

Form

Supplied as a lyophilized powder from 20mM phosphate buffer, 150mM sodium chloride, pH 7.4. No preservative added. Reconstitute with 1.31ml distilled water.

Purity

(same/more than)95% pure (SDS-PAGE).