Transferrin, Human, Iron-Free (APO)

Transferrin is the iron-transport protein of vertebrate serum and donates iron to cells through interaction with a specific membrane receptor, CD71. Transferrin appears to be indispensable for most cells growing in tissue culture. It is referred to frequently as a growth factor because, in analogy to other growth factor-receptor interactions, proliferating cells express high numbers of transferrin receptors, and the binding of transferrin to their receptors is needed for cells to initiate and maintain their DNA synthesis. Apart from its role as an iron transport protein transferrin acts as a cytokine and has functions that may not be related to its iron-carrying capacity Transferrin has been identified also as one of the factors promoting the clonal growth of murine granulocyte and macrophage precursors cultured in vitro under serum-free conditions.\n\nHuman ApoTransferrin is a glycoprotein of ~77kD containing 698 amino acids. \n\nHuman Virus Test:\nFDA approved Plasma from each donor has been tested and found negative for antibody to HIV-1, HIV-2, HCV, HBSAG, HBc, ALT and Syphilis. Viral inactivation by pasteurization (60 degrees C for 10 hours) has been validated using three different test viruses, with removal of 8-14.5 logs of virus documented. The purification process has also been found to remove significant additional quantities of virus.\n\nApplications:\nSuitable for use in in-vitro cultivation of mammalian cells, in-vitro long-term cells growth, detoxificant in media by binding contaminating metal ions, as a fermentation nutrient and affinity purification of anti-human transferrin antibodies and as a receptor mediated transfection of molecules such as DNA, into cells. Other applications not tested.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months after receipt at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8205-47

Size

500mg

Form

Supplied as a lyophilized powder. No additives. Reconstitute with sterile ddH20 to (same/more than)0.1mg/ml.

Purity

~97% as determined by Cellulose Acetate.

References

1. Garcia C, Paez P, Soto EF, J Neurosci Res 2004 Nov 1;78(3):338-46 2. Xiu-Lian D, Kui W, Lan Y, Zhong Ming Q, J Cell Physiol 2004 Oct;201(1):45-54 3. Heikkinen J, Koskenkari J, Dahlbacka S, Hirvonen J, Scand Cardiovasc J 2004 Jun;38(3):178-86 4. Paez PM, Garcia CI, Campagnoni AT, Glia 2004 Apr 15;46(2):207-17 5. [FEMS Immunol. Med Microbiol. 37 (2003) 45-51]. von Bonsdorff L, Sahlstedt L, Ruutu T, FEMS Immunol Med Microbiol 2004 Mar 8;40(2):173-80 6. de Vries B, Walter SJ, Wolfs TG, Transplantation 2004 Mar 15;77(5):669-75.