Transferrin, Human (Tf) Control Peptide

Elemental iron is required for a variety of normal cellular functions and vital for proper growth and development. However, natural iron is quite insoluble and excess iron is harmful, since it can catalyze the formation of potentially damaging reactive oxygen species. The major pool of body iron (~85%; 40-50mg/kg) is found in circulating hemoglobin and muscle myoglobin. Iron absorption occurs primarily in the intestine (duodenum) and inversely related to body iron reserve. Several proteins including Ferritin, transferrin (Tf), transferrin receptors (TfRs), and iron regulatory proteins (IRPs) etc play a key role in iron metabolism.\n\nTransferrin (Tf, human chromosome 3, 679 aa), a serum glycoprotein of ~80kD and synthesized in the liver, is the primary protein of inter-organ transport of nonheme iron. Tf can bind two iron atoms. Tf binds to membrane Transferrin receptors (TfRs) and taken up by endocytosis. Iron is released from Tf, within acidic endosomes, into the cytoplasm apparently through the action of DMT1. The apoTf-TfR complex is returned to the cell surface, where, apo-Tf dissociates from TfR at the extracellular pH. The classical TfR, now termed TfR1, is a homodimeric (95kD subunits) type II membrane glycoprotein that binds two molecules of Tf. Human TfR1 (human 760 aa; mouse 763 aa) has a cytoplasmic domain 1-67aa, 68-88 aa TM, and 89-760 aa as extracellular domains. A monomeric serum form or soluble TfR1 (~80kD) also exists that lacks residues 1-100 aa. Recently, a second Tf receptor, TfR2, has been cloned and characterized. TfR2 shares 45% identity with TfR1, and 27% with PMSA. Several variants of Tf have been identified with varying iron binding ability.\n\nApplications: Suitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: Ready to use (10 ul/lane)\nELISA: Coat ELISA plates at 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8199-10

Size

100ul

Applications

ELISA, WB

References

Bowman, B. H. et al (1988) Adv. Genet. 25: 1-38; Evans, R. W. et al (1982) Biochem. J. 201: 19-26; MacGillivray, R. T. A et al (1982) PNAS 79: 2504-2508; Park, I. et al (1985) PNAS 82, 3149-3153; Uzan, G. et al (1984) BBRC 119, 273-281; Yang, F. et al (1984) PNAS 81, 2752-2756; Nelson N et al (1999) EMBO J. 18, 4361(review); Cairo G et al (2000) Biochem. J. 352, 241-250.