Transferrin Receptor 1, Human, Western Blot Positive Control (TfR1)

Western Blot positive control for T8199-42 and T8199-46.\n\nElemental iron is required for a variety of normal cellular functions and vital for proper growth and development. Transferrin (Tf), a serum glycoprotein of ~80kD and synthesized in the liver, is the primary protein of inter-organ transport of nonheme iron. Tf can bind two iron atoms. Tf binds to membrane Transferrin receptors (TfRs) and taken up by endocytosis. Iron is released from Tf, within acidic endosomes, into the cytoplasm apparently through the action of DMT1. The apoTf-TfR complex is returned to the cell surface, where, apo-Tf dissociates from TfR at the extracellular pH. The classical TfR, now termed TfR1, is a homodimeric (95kD subunits) type II membrane glycoprotein that binds two molecules of Tf. Human TfR1 (human 760aa; mouse 763aa) has a cytoplasmic domain 1-67aa, 68-88aa TM, and 89-760aa as extracellular domains. A monomeric serum form or soluble TfR1 (~80kD) also exists that lacks residues 1-100aa. A second Tf receptor, TfR2, has been cloned and characterized. TfR2 shares 45% identity with TfR1, and 27% with PMSA. Human TfR2 (human alpha 801aa, Chromosome 7q22; mouse alpha 798aa) is predicted to contain a cytoplasmic domain of 1-80aa, 1 TM domain followed by 105-801aa as the extracellular domain. It is highly expressed in liver and peripheral blood mononuclear cells. In contrast to Tfr1, expression of Tfr2 is not down regulated as a result of iron overload, consistent with the absence iron-responsive element in TfR2. It is alternatively spliced to alpha and beta isoforms. TfR2-beta protein lacked the N-terminal portion of the TfR2-alpha including the putative TM domain.\n\nApplications:\nSuitable for use in Western Blot only.\n\nRecommended Dilutions:\nWestern Blot: 10ul per lane.\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T8199-43

Size

100ul

Applications

WB

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Purity

Purified using Protein A/G column chromatography (~95%).

References

1. Schneider, C., et al., Nature 311: 675-678 (1984). 2. McClelland, A., et al., Cell 39: 267-274 (1984). 3. Shih, Y.J., et al., JBC 265: 19077-19081 (1990). 4. Nelson, N., et al., EMBO J. 18: 4361-4371 (1999, review). 5. Cairo, G., Pietrangelo, A., et al., Biochem. J. 352: 241-250 (2000).