Transferrin Receptor (CD71) (FITC)

Transferrin receptor is a 190kD Type II membrane glycoprotein which consists of two subunits of 95kD. It is expressed on activated lymphocytes, macrophages and monocytes. It binds Fe (Apo) transferrin. The gene coding for the antigen is located on the q-arm of chromosome 3. The ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias and lymphomas. Transferrin receptors are also present on reticulocytes, but are lost during maturation into erythrocytes. The function of the transferrin receptor is believed to be mainly nutritional. Alternatively, it has been suggested that a growth signal might be provided by the interaction of transferrin and transferrin receptor. CD71 is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro. The transferrin receptor has been structurally characterized as a disulfide-bound dimer of identical glycoprotein subunits of 95kD. The transferrin receptor is not present on resting peripheral blood lymphocytes, but appears after mitogenic stimulation on B and T cells. Erythroblasts express high levels of CD71. It is also present on macrophages. It is expressed by some T-and B-cell lines. CD71 expression is linked to cell proliferation and intracellular iron concentration. It has been studied at the Vth International Workshop on Human Leukocyte Differentiation Antigens, Boston (1993).\n\nApplications: \nSuitable for use in Flow Cytometry, Immunoprecipitation and In vivo and In vitro inhibition of cell proliferation. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: (same/less than)1ug /10e6 cells \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8199-25

Size

500ug

Applications

FC, IP

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a,k

References

1. Lesley, J., et al., Immunogenetics 15: 313 (1982). 2. Lesley, J., et al., Mol. Cell. Biol. 4(9): 1675 (1984). 3. Kemp, J.D., et al., J. Immunol. 138: 2422 (1987). 4. Kemp, J.D., et al., Cell Immunol. 122: 218 (1989). 5. Sauvage, C.A., et al., 1987, Cancer Res. 47:747.

Additional Info

Recognizes mouse CD71 transferrin receptor, Mr180-190kD (90-95kD under reducing conditions).