Transferrin Receptor, NT (CD71, TR, Tfr, TfnrR)

Human transferrin receptor (TR) is a homodimeric type II membrane protein consisting of two identical 95kD subunits covalently linked by intermolecular disulfide bonds. The TR has an extracellular domain of 671 amino acids, a single 28 residue transmembrane region and a 61 residue amino terminal cytoplasmic domain. Expression of the receptor is limited on normal tissue, however, highly expressed on the surface of some tumors. TR is included in the class of ligand transport receptors which are internalized and clustered in coated pits. An important function of the receptor is to bind the serum transport protein transferrin (TF) and mediate the uptake of iron into the cell. A growing area of research involves the use of monoclonal anti-TF receptor antibodies to inhibit the uptake of iron and thus block tumor cell growth. \n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry, Electron Microscopy. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1

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SPECIFICATIONS

Catalog Number

T8199-41

Size

250ug

Applications

ELISA, ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Ch/Bird

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG1\n

References

1. White S, et al. 1992. Biochemica et Biophysica Acta:1136. 2. White S, et al. 1990. Cancer Research:50.

Additional Info

Recognizes the N-terminus region of the human transferrin receptor. Due to the conserved amino acid sequences of the cytoplasmic tail among species, this antibody is able to crossreact with chicken, mouse and Chinese hamster TR(1). Crossreactivity has also been observed with rat adrenal pheochromocytoma (PC-12) (1). Inhibits the internalization of transferrin receptors.