Transferrin, also known as serotransferrin and siderophilin, is an 80kD secreted glycoprotein that binds two Fe (3+) ions, transporting them from sites of absorption and heme degradation to those of storage and utilization. It is a member of the Transferrin family of proteins. Human Transferrin is synthesized as a 698 amino acid (aa) precursor that contains a 19aa signal sequence and a 679aa mature chain. The mature chain consists of two Transferrin-like domains, two high-affinity iron binding sites, one potential site for O-linked glycosylation, and two potential sites for N-linked glycosylation. Human Transferrin has sequence homology of 73% to mouse and rat Transferrin.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 2ug/ml with the appropriate secondary reagents to detect human Transferrin under non-reducing conditions only. Lysates were prepared from human liver tissue. PVDF membrane was probed with 2ug/ml of T8199-01B followed by HRP
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