TGFb1 (Transforming Growth Factor-beta1) is a family of multifunctional 25kD proteins. TGFb1 was originally identified for its ability to induce the growth of normal rodent fibroblasts in soft agar. It is now known that TGFb1 is a potent growth inhibitor of many normal and transformed cell lines. It regulates normal cell growth, development and tissue remodeling following injury. TGFb1 is produced as latent high molecular weight complexes. The activation of latent TGFb1 is an important step in the regulation of its action.
Applications:
Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions:
ELISA: 1:2000-1:10,000
Western Blot: 1:200-1:1000
Immunohistochemistry (Paraffin): 5ug/ml
Immunofluorescence: 1:2000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.