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Transforming Growth Factor beta 1 (TGFb1, TGF-beta-1, TGFB1, TGFB)

Transforming Growth Factor beta 1 (TGFb1, TGF-beta-1, TGFB1, TGFB)

Cat no: T8250-16E

Supplier: United States Biological
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TGFb1 (Transforming Growth Factor-beta1) is a family of multifunctional 25kD proteins. TGFb1 was originally identified for its ability to induce the growth of normal rodent fibroblasts in soft agar. It is now known that TGFb1 is a potent growth inhibitor of many normal and transformed cell lines. It regulates normal cell growth, development and tissue remodeling following injury. TGFb1 is produced as latent high molecular weight complexes. The activation of latent TGFb1 is an important step in the regulation of its action. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells in 100ul. Membrane permeabilization is required. Immunohistochemistry (Paraffin, Frozen): Does not require protein digestion pre-treatment of paraffin sections. Paraformaldehyde fixation is recommended. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-04D: IgG, H&L (AP) Pab Gt xMo I1904-06F: IgG, H&L (FITC) Pab Gt xMo I1904-06C: IgG, H&L (HRP) Pab Gt xMo Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control IHC Postivie Control: Human Breat Carcinoma Hybridoma: SP2/0-Ag 14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T8250-16E
Reactivities: Human, Mouse, Rat, Bovine, Rabbit, Sheep
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by affinity chromatography.
References: 1. Lehr, E.J.et al. (2010) Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches. J Thorac Cardiovasc Surg. Jul 14. [Epub ahead of print] 2. Westermann, D. et al. (2011) Circ Heart Fail. 4: 44
Additional info: Recognizes human platelet-derived and recombinant Transforming Growth Factor beta 1. Demonstrated to react with dimeric (25kD) or monomeric (12.5kD) molecules of natural TGFb1 under non-recducing and reducing conditions respectively. Species Crossreactivity: rat, sheep, mustelid, rabbit, mouse, bovine and mink

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