Transforming Growth Factor beta 1 (TGFb1, TGF-beta-1, TGFB1, TGFB)

TGFb1 (Transforming Growth Factor-beta1) is a family of multifunctional 25kD proteins. TGFb1 was originally identified for its ability to induce the growth of normal rodent fibroblasts in soft agar. It is now known that TGFb1 is a potent growth inhibitor of many normal and transformed cell lines. It regulates normal cell growth, development and tissue remodeling following injury. TGFb1 is produced as latent high molecular weight complexes. The activation of latent TGFb1 is an important step in the regulation of its action.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 10ul labels 1x10e6 cells in 100ul. Membrane permeabilization is required.\nImmunohistochemistry (Paraffin, Frozen): Does not require protein digestion pre-treatment of paraffin sections. Paraformaldehyde fixation is recommended.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-04D: IgG, H&L (AP) Pab Gt xMo\nI1904-06F: IgG, H&L (FITC) Pab Gt xMo\nI1904-06C: IgG, H&L (HRP) Pab Gt xMo\n\nRecommended Negative Control:\nI1904-78R: IgG1 Murine Negative Control\n\nIHC Postivie Control:\nHuman Breat Carcinoma\n\nHybridoma: \nSP2/0-Ag 14 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8250-16E

Size

100ug

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Rabbit, Sheep

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG1

References

1. Lehr, E.J.et al. (2010) Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches. J Thorac Cardiovasc Surg. Jul 14. [Epub ahead of print] 2. Westermann, D. et al. (2011) Circ Heart Fail. 4: 44

Additional Info

Recognizes human platelet-derived and recombinant Transforming Growth Factor beta 1. Demonstrated to react with dimeric (25kD) or monomeric (12.5kD) molecules of natural TGFb1 under non-recducing and reducing conditions respectively. Species Crossreactivity: rat, sheep, mustelid, rabbit, mouse, bovine and mink