TGF-beta is secreted by cells in the form of an inactive complex. This complex consists of TGF-beta associated non-covalently with a protein designated the latency associated peptide (LAP). TGf-beta and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion. LAP and TGF-beta each consist of a disulfide-linked homodimer and the association of these two components renders TGF-beta inactive and inaccessible to anti-TGF-beta antibodies.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing PerCP conjugates will result in a substantial loss of activity. PerCP conjugates are sensitive to light.