Transforming Growth Factor, beta-induced, 68kDa (Transforming Growth Factor-beta-induced Protein ig-h3, Beta ig-h3, BIGH3, TGFBI, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato-epithelin, LCD1, RGD-containing Collagen-associated Protein, RGD-CAP)

BIG-H3 (TGFBI/RGD-CAP/Kerato-epithelin) is a 683aa secretory protein induced by TGF-B that plays a role in cell adhesion, differentiation, and apoptosis (1-4). BIG-H3 contains an internal cysteine-rich EMI domain followed by four fasciclin-1 domains and a carboxy terminal RGD domain (1,2). It contributes to cell adhesion through interactions with integrins as well as a number of extracellular matrix (ECM) proteins including collagen, fibronectin, and laminin (5-7). ECM BIG-H3 is found in a wide variety of tissues (8-12). Mutations in the BIG-H3 gene\nas well as elevated protein levels are most notably associated with corneal dystrophies (13).\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence (IF-IC): 1:800\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T8252-91H

Size

100ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

(1) Skonier, J. et al. (1992) DNA Cell Biol 11, 511-22. (2) Skonier, J. et al. (1994) DNA Cell Biol 13, 571-84. (3) Hashimoto, K. et al. (1997) Biochim Biophys Acta 1355, 303-14. (4) Kim, J.E. et al. (2003) Oncogene 22, 2045-53. (5) Kim, J.E. et al. (2002) Invest Ophthalmol Vis Sci 43, 656-61.\n(6) Billings, P.C. et al. (2002) J Biol Chem 277, 28003-9. (7) Hanssen, E. et al. (2003) J Biol Chem 278, 24334-41. (8) Gibson, M.A. et al. (1997) J Histochem Cytochem 45, 1683-96. (9) Billings, P.C. et al. (2000) Am J Respir Cell Mol Biol 22, 352-9. (10) Gilbert, R.E. et al. (1998) Kidney Int 54, 1052-62.\n(11) Rawe, I.M. et al. (1997) Invest Ophthalmol Vis Sci 38, 893-900. (12) LeBaron, R.G. et al. (1995) J Invest Dermatol 104, 844-9. (13) Munier, F.L. et al. (1997) Nat Genet 15, 247-51.

Additional Info

Recognizes endogenous levels of total BIG-H3.