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Transforming Growth Factor beta Induced (BIGH3, TGFBI, Beta-ig-h3)

Cat no: T8252-91

Transforming Growth Factor beta Induced (BIGH3, TGFBI, Beta-ig-h3)

Beta-IG-H3 (TGFBI/RGD-CAP/Kerato-epithelin) is a 683-amino acid secretory protein induced by TGF-b that plays a role in cell adhesion, differentiation, and apoptosis (1-4). Beta-IG-H3 contains an internal cysteine-rich EMI domain followed by four fasciclin-1 domains and a carboxyl terminal RGD domain (1,2). It contributes to cell adhesion through interactions with integrins as well as a number of extracellular matrix (ECM) proteins including collagen, fibronectin, and laminin (5-7). ECM Beta-IG-H3 is found in a wide variety of tissues (8-12). Mutations in the Beta-IG-H3 gene as well as elevated protein levels are most notably associated with corneal dystrophies (13). \n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (IF-IC): 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8252-91

Size

100ul

Applications

ICC, IF, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Skonier, J., et al., DNA Cell Biol. 11: 511

Additional Info

Recognizes endogenous levels of total human Beta-IG-H3 protein. Species Sequence Homology: monkey

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