Transforming Growth Factor beta (TGFb)

Transforming growth factor-beta (TGF-beta) family of proteins has potent hematopoietic regulatory properties ranging from effects on the growth and differentiation of primitive stem cells to the differentiated functions of mature cells.\n\nApplications: \nSuitable for use in Western Blot, ELISA, Neutralization and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 5-20ng/ml will allow visualization of 100ng/lane of TGF-beta. Recognizes the dimeric (25kD) and monomeric (12.5kD) forms of TGF-beta under both non-reducing and reducing conditions respectively.\nNeutralization: Neutralizes TGF-beta activity in vitro in an inhibition assay of CCL/64 cell growth and the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo.\nImmunohistochemistry (formalin-fixed, paraffin-embedded, frozen): 1:1000. Has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. \nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8250-12A

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.0.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Crawford, S.E., Thrombospondin-1 is a major activator of TGF-beta in vivo. Cell 93: 1159-1170 (1998). 2. Biological characterization of a monoclonal antibody TB21 against human Transforming Growth Factor-Beta. Aeta Biologiac Experimentalis Sinica 26 (1993).

Additional Info

Recognizes both human platelet-derived and recombinant TGF-beta.