Transforming Growth Factor beta1, beta2, beta3 (TGFb1, TGFb2, TGFb3) (APC)

Transforming Growth Factor Beta (TGFb) is a stable, multifunctional polypeptide growth factor. While specific receptors for this protein have been found on almost all mammalian cell types thus far examined, the effect of the molecule varies depending on the cell type and growth conditions. Generally, TGFb is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodermal origin. \nTGFb1, 2, and 3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGFb1, 2, and 3 are disulfide-linked homodimers.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: Neat. Use 10ul to label 5x10e5 cells. \nFor intracellular staining, cells must be fixed with 4% paraformaldehyde in PBS and permeabilized with 0.1% saponin in a balanced salt solution to facilitate antibody entrance into cells. Saponin must be used in all buffers used (both staining and washing steps).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing Allophycocyanin conjugates will result in a substantial loss of enzymatic activity.

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SPECIFICATIONS

Catalog Number

T8250-19A

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum, Mouse, Bov, Ch/Bird

Form

Supplied as a liquid in PBS, (same/less than)0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Ayala, A., et al., FASEB J. 6: A1604 (1992). 2. Roberts, A.B. & Sporn, M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer

Additional Info

Recognizes bovine TGFb1 and TGFb2. Species Crossreactivity: mouse TGFb1 and TGFb2; human TGFb1, recombinant human TGFb2 and recombinant human TGFb3; recombinant chicken TGFb3; recombinant avian TGFb5.