logo
logo
Transglutaminase II (BSA & Azide Free)

Cat no: T8260-02X

Transglutaminase II (BSA & Azide Free)

Transglutaminase II catalyzes calcium-dependent post-translational modification of proteins by formation of an isopeptide bond within or between polypeptide chains. It is also known as TGC, tTG, type II-, Gh, cytosolic-, liver-, endothelial-, erythrocyte-, cellular-transglutaminase. Different tissues and cell types express varying amounts of tissue transglutaminase with a markedly high expression in rheumatoid lesions. It is implicated in programmed cell death, signal transduction, drug resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing. \n\nMW of Antigen: 77-85kD\n\nApplications and Recommended Dilutions: \nImmunoprecipitation (Native and denatured) (Use Protein G) (Ab 2ug/mg protein lysate) \nWestern Blotting (Ab 0.5-1ug/ml for 2hrs at RT) \nImmunohistochemistry (Formalin/paraffin) (Ab 1-2ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator. \n\nPositive Control: HUVEC cells. Breast carcinoma. \n\nCellular Localization: Cytoplasm, cell membrane, and extracellular matrix\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degrees C or colder. Aliquots are stable for at least 12 months at-20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T8260-02X

Size

100ul

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, GP

Form

Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See T8260-02

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Birckbichler PJ, et. al. Hybridoma, 1985, 4:179

Additional Info

Recognizes Transglutaminase II. Species Crossreactivity: human and guinea pig.

Read more on Supplier website
Advertisehere
MtoZ Horizontal banner
MtoZ Biolabs

Latest promotion FROM:

MtoZ Biolabs
View promotion

Latest promotions

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...