Transglutaminase II

Transglutaminase II catalyzes calcium-dependent post-translational modification of proteins by formation of an isopeptide bond within or between polypeptide chains. It is also known as TGC, tTG, type II-, Gh, cytosolic-, liver-, endothelial-, erythrocyte-, cellular-transglutaminase. Different tissues and cell types express varying amounts of tissue transglutaminase with a markedly hugh expression in rheumatoid lesions. It is implicated in programmed cell death, signal transduction, drug resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing. \n\nApplications:\nSuitable for use in Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry, Affinity Purification and Biological Blockade. Other applications have not been tested.\n\nRecommended Dilution: \nAffinity Purification: Use without BSA \nBiological Blockade: Use without azide \nImmunoprecipitation (Use Protein G): 2ug/mg protein lysate. \nWestern Blot: 1-2ug/ml for 2hrs at RT\nImmunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT.\nNote: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHUVEC cells. Breast carcinoma. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

T8260-04

Size

500ul

Applications

IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Can, GP, Rabbit

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T8260-04X

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Birckbichler PJ, et al., Hybridoma 4:179-86 (1985).

Additional Info

Recognizes guinea pig Transglutaminase II. Inhibits the TGase II activity. Species Crossreactivity: human, canine, rabbit. Does not react with mouse and rat.