Transglutaminase, Tissue, Recombinant, Human (tTG)

Celiac disease is an enteropathy that is characterized by intestinal lesions of variable severity. Tissue-type transglutaminase (tTG) is believed to be the predominant autoantigen for celiac disease and the corresponding autoantibodies show higher sensitivity and specificity than anti-gliadin antibodies. Highly pure recombinant human tTG is now available to replace the traditionally used tTG fraction from guinea pig. Tissue-type transglutaminase antigens have been specifically modified for improved handling: exchange of an active site amino acid eliminates the protein cross-linking activity of the enzyme, while maintaining the native three-dimensional structure and the enzyme's secondary GTPase activity. This engineering assures reproducible properties of the antigen preparations through the absence of variable and ill-defined covalent aggregates of tTG antigen and host cell proteins.\n\nRecombinant Human Tissue Transglutaminase produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 78.02kD. rHutTG is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. By point mutation of the active center the catalytic transglutaminase activity has been eliminated, resulting in increased stability during storage and coating.\n\nApplications: \nSuitable for use in Western Blot with monoclonal anti-hexa-His-tag antibody and standard ELISA (checker-board analysis of positive/negative sera panels, immuno-dot test). Also suitable for biotinylation and iodination. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.6-1.4ug/ml (depending on the type of plate and coating buffer). Optimal dilutions to be determined by the researcher.\n\nSpecificity: \nBinds IgG-type human auto-antibodies.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T8259-98

Size

5ug

Form

Supplied as a liquid in 16mM HEPES buffer, pH 7.6, 240mM sodium chloride and 20% glycerol.

Purity

(same/more than)95% (SDS-PAGE)

References

1. Mol Cancer Ther 5(6):1493-1503 (June 2006). 2. Aliment Pharmacol Ther 24(1):47-54 (July 2006) 3. Adv Anat Pathol 13(3):141-2 (May 2006) 4. Proc Natl Acad Sci U S A 103(24):9023-8 (Jun 132006) 5. Protein J 25(1):83-94 (2006).