Transporter Associated Protein (TAP1, Transporter Associated with Antigen Processing, ABC Transporter MHC-1, ABC17, Antigen Peptide Transporter 1, APT1, ATP Binding Cassette Sub-family B (MDR/TAP) Member 2, ATP Binding Cassette Sub-family B Member 2, ABCB

TAP1, a 70kD transmembrane protein, and TAP2 are two structurally related subunits of the transporter associated with antigen processing (TAP). The TAP complex is a member of the ATP binding cassette (ABC) family of transmembrane transporters. TAP1 and TAP2 each contain an N-terminal transmembrane region and C-terminal nucleotide binding domains (NBD). TAP1 and TAP2 form a complex in the endoplasmic reticulum (ER) membrane with the NBD oriented in the cytosol. The TAP transporter is an essential component of the MHC class I antigen presentation pathway by binding peptides in its cytosolic part and subsequently translocating the peptides into the lumen of the endoplasmic reticulum (ER) where assembly of MHC class I and pepide takes place. Assembly of MHC class I-beta2-micorglobulin (beta2-m) dimers in the ER involves 2 chaperones, calnexin which interacts with free class I heavy (H) chains and calreticulin which binds human class I-beta2 dimers prior to peptide loading. Calreticulin remains associated with at least a subset of class I molecules when they in turn bind to TAP. Polymorphic differences in MHC class I H chains can results in quantitative as well as qualitative differences in how they interact with peptide, beta2-m, calnexin, calreticulin, ERp57, TAP and Tapasin, a subunit of the TAP complex which binds to both TAP1 and MHC class I. Data obtained with Tapasin deletion mutants revealed that binding to TAP is mediated by the C-terminal region and that the N-terminal region is required to stabilize the MHC class I loading complex. The Tapasin gene is centromeric of HLA-DP locus between the HSET and HKE1.5 genes and within 500 kbp of the transporters associated with antigen processing, TAP1 and TAP2 genes. The localization of these genes within such a short distance of each other on the chromosome implies some regulatory or functional significance.\n\npplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000\nWestern Blot: 1:1,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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