$TRAP 5 (Tartrate-resistant Acid\nPhosphatase Serum band 5, TRAcP 5, ACP5, PAP, EC 3.1.3.2) (Biotin)$

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Cat no: T8266-97B

TRAP 5 (Tartrate-resistant Acid\nPhosphatase Serum band 5, TRAcP 5, ACP5, PAP, EC 3.1.3.2) (Biotin)

TRAP 5 (serum band 5 tartrate-resistant acid phosphatase, TRACP 5; EC 3.1.3.2) is a glycoprotein of 35-37kD. TRAP 5 belongs to the most abundant enzymes in osteoclasts. It is expressed in certain differentiated cells of the mononuclear phagocyte system, particularly osteoclasts and alveolar macrophages, where it takes an active part in the bone resorption process. High blood levels of TRAP 5 are usually associated with active bone remodeling. Increased serum levels are observed during normal bone growth among healthy children. Elevated serum TRAP levels have been detected in diseases characterized by increased bone resorption; Paget's disease of the bone, hemodialysis, primary hyperparathyroidism, metastatic malignancies involving bone resorption, multiple myeloma, and bilaterally ovariectomized women. Post-menopausal women have higher levels of serum than postmenopausal women on estrogen replacement therapy. Therefore, specific determination of TRAP 5 activity can be essential for clinical assessment of bone metabolism. \n\n\nApplications:\nSuitable for use in Western blot. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern blot: 0.1-0.2ug/ml. The detection limitis~10ng/lane under non-reducing and reducing conditions. \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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