$TRAP 5 (Tartrate-resistant Acid\nPhosphatase Serum Band 5, TRAcP 5, ACP5, PAP, EC 3.1.3.2)$

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Cat no: T8266-97

TRAP 5 (Tartrate-resistant Acid\nPhosphatase Serum Band 5, TRAcP 5, ACP5, PAP, EC 3.1.3.2)

TRAP 5 (serum band 5 tartrate-resistant acid phosphatase, TRACP 5; EC 3.1.3.2) is a glycoprotein of 35-37kD. TRAP 5 belongs to the most abundant enzymes in osteoclasts. It is expressed in certain differentiated cells of the mononuclear phagocyte system, particularly osteoclasts and alveolar macrophages, where it takes an active part in the bone resorption process. High blood levels of TRAP 5 are usually associated with active bone remodeling. Increased serum levels are observed during normal bone growth among healthy children. Elevated serum TRAP levels have been detected in diseases characterized by increased bone resorption; Paget's disease of the bone, hemodialysis, primary hyperparathyroidism, metastatic malignancies involving bone resorption, multiple myeloma and bilaterally ovariectomized women. Post-menopausal women have higher levels of serum than postmenopausal women on estrogen replacement therapy. Therefore, specific determination of TRAP 5 activity can be essential for clinical assessment of bone metabolism. \n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8266-97

Size

100ug

Applications

ELISA

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophillized powder from PBS, pH 7.2. No preservatives added. Reconstitute with100ul sterile ddH2O.

P Type

Mab

Purity

Purified by Portein G affinity chromatography.

Isotype

IgG1

References

1. Nakanishi, M., et al., Clin Chem. 44: 221-225 (1998). 2. Halleen, J.M., et al., J. Bone Miner. Res. 15: 133-1345 (2000). 3. Lamp, E.C. & Drexler, H.G., Leuk. Lymphoma 39: 477-484 (2000). 4. Waguespack, S.G., et al., J. Clin. Endocrinol. Metab. 87: 2212-2217 (2002). 5. Igarashi, Y., et al., J. Chromatogr. B. 781: 345-358 (2002). 6. Terpos, E., et al., Int. J. Cancer 106: 455-457 (2003).

Additional Info

Recognizes human TRAP 5.