TRBP2 (Trans-activation Responsive RNA Binding Protein 2, Tar (HIV-1) RNA Binding Protein 2, TAR RNA-binding Protein 2, TARBP2, TRBP, LOQS, RISC-loading Complex Subunit TARBP2)

TRBP belongs to the family of dsRNA-binding proteins. It consists of highly conserved 2 dsRNA-binding domains and C-terminal basic region. It is a unique cellular protein capable of binding to HIV-TAR RNA and stimulates expression of HIV-1 long terminal repeats. It also inhibits the host antiviral and anti-proliferative mechanisms by directly binding to IFN-regulated dsRNA-dependent protein kinase (PKR). TRBP associates with Ago2 protein and forms an integral component of Dicer-containing complex. It has a role in micro RNA processing and in RISC assembly. Other functions of TRBP include promoting cellular growth and tumorigenesis.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nBrain lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8269-01C

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, ZF/Fish

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Battisti,P.L., Daher,A., Bannwarth,S., Voortman,J., Peden,K.W., Hiscott,J., Mouland,A.J., Benarous,R. and Gatignol,A. Additive activity between the trans-activation response RNA-binding protein, TRBP2, and cyclin T1 on HIV type 1 expression and viral production in murine cells AIDS Res. Hum. Retroviruses 19 (9), 767-778 (2003) 2. Daher,A., Longuet,M., Dorin,D., Bois,F., Segeral,E., Bannwarth,S., Battisti,P.L., Purcell,D.F., Benarous,R., Vaquero,C., Meurs,E.F. and Gatignol,A. Two dimerization domains in the trans-activation response RNA-binding protein (TRBP) individually reverse the protein kinase R inhibition of HIV-1 long terminal repeat expression J. Biol. Chem. 276 (36), 33899-33905 (2001)

Additional Info

Recognizes human TRBP2. Species Crossreactivity: bovine, chimpanzee, mouse, rat, zebrafish.

Alternative Names

Trans-activation-responsive RNA-binding Protein