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Trefoil Factor 2 (TFF2, SML1, Spasmolysin, Spasmolytic Polypeptide, SP)

Cat no: T8269-74F


Supplier: United States Biological
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Human spasmolytic polypeptide (HSP) is a trefoil peptide found prominently in the foveolar and surface epithelium, pyloric glands and mucous neck cells of the stomach. This antibody will help detect HSP in normal and uncharacterized tissues. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:25-1:50 for 1 hr at RT Optimal dilutions to be determined by the researcher. Positive Control: Stomach or duodenum (cytoplasmic staining pattern) Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: T8269-74F
Reactivities: Human
Hosts: Mouse
Applications: Immunohistochemistry
Size: 1ml
Form: Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
P type: Mab
Isotype: IgM
Purity: Supernatant
References: 1.Playford R J, Marchbank T, Chinery R, et al.. Human spasmolytic polypeptide is a cytoprotective agent that stimulates cell migration. Gastroenterology. 108:108-116 (1995). 2.Elia G, Chinery R, Hanby A M, et al.. The production and characterisation of a new monoclonal antibody to the trefoil peptide human spasmolytic polypeptide. Histochemical Journal. 26:644-647 (1994). 3.Thim L. Trefoil peptides: A new family of gastrointestinal molecules. Digestion. 55:353-360 (1994). 4.Hanby A M, Poulsom R, Singh S, et al.. Spasmolytic polypeptide is a major antral peptide: Distribution of the trefoil peptides human spasmolytic polypeptide and pS2 in the stomach. Gastroenterology. 105:1110-1116 (1993). 5.Poulsom R and Wright N A. Trefoil peptides: a newly recognized family of epithelial mucin-associated molecules. American Physiological Society. Invited Review. G205-G213 (1993).
Additional info: Recognizes Human Spasmolytic Polypeptide.

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