TREM-2b (Trem2b, Triggering Receptor Expressed on Monocytes 2) (PE)

TREM-2 is an Ig superfamily cell surface receptor that activates a number of myeloid cell types. It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC. A single human TREM-2 gene has been described, however, two closely related orthologs were reported in mouse. The proteins differ by only three amino acids and were designated TREM-2a and TREM-2b. TREM-2 is type I transmembrane protein consisting of a single extracellular immunoglobulin (V-like) domain, a transmembrane domain with a positively charged lysine residue, and a short cytoplasmic tail. It associates with the signal adapter protein, DAP12, for signaling and function. DAP12 has a cytoplasmic ITAM that is phosphorylated upon ligand binding leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM-2 is expressed by immature monocyte-derived dendritic cells (DC), and expression is down-regulated upon activation of DC by microbial products and costimulatory signals. Ligation of TREM-2 on immature DC with anti-TREM-2 antibodies results in partial DC activation and the up-regulation of CCR7 and some co-stimulatory molecules. A role for TREM-2 in the functioning of osteoclasts and microglia is suggested by the discovery that homozygous loss-of-function mutations in either TREM-2 or DAP12 result in Nasu-Hakola disease characterized by a combination of presenile demetia and bone cysts. In vitro studies indicate that the differentiation of myeloid precursors into osteoclasts is dramatically impaired in TREM-2 deficient individuals.\n\nApplications: \nSuitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: Neat. Use 10ul to label 1x10e5 cells.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

T8282-10B

Size

100Tests

Applications

FC, ICC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with PE.

P Type

Mab

Purity

Purified by Protein G affinity chromatography prior to conjugation.

Isotype

IgG2b

References

1. Colonna, M., Nature Rev. Immunol. 3: 445 (2003). 2. Allcock, R., et al., Eur. J. Immunol. 33: 567 (2003). 3. Daws, M., et al., Eur. J. Immunol. 31: 783 (2001). 4. Bouchon, A., et al., J. Exp. Med. 194: 1111 (2001). 5. Paloneva, J., et al., Am. J. Hum. Genet. 71: 656 (2002). 6. Cella, M., et al., J. Exp. Med. 198: 645 (2003).

Additional Info

Recognizes mouse TREM-2b.