TREML1 is a type I transmembrane protein that differs from TREM in the cytoplasmic region by having two ITIMs (Immunoreceptor Tyrosine-based Inhibition Motif). A splice variant lacking ITIMs in its cytoplasmic domain also exists. TREML1 is found only in platelets and megakaryocytes. The extracellular region of human and mouse TLT-1 share 75% amino acid sequence identity.
Applications:
Suitable for use in Western Blot and Direct ELISA. Other applications not tested.
Recommended Dilution:
Western Blot: 1-2ug/ml Detection limit for recombinant mouse TREML1 is is ~25 ng/lane under non-reducing conditions. Reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5-50 fold.
Direct ELISA: 0.5-1.0ug/ml .Detection limit for recombinant mouse TREML1 is ~1ng/well.
Optimal dilutions to be determined by the researcher.
Hybridoma:
Fusion of mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse TREM-Like transcript 1 (rmTREML1) extracellular domain.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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